path protein microarray slides (Grace Bio-Labs)
86
Structured Review
Grace Bio-Labs
path protein microarray slides
Path Protein Microarray Slides, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+microarray/microarray+path+protein+slides/pmc12455989-242-62-66
Average 86 stars, based on 1 article reviews
Path Protein Microarray Slides, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+microarray/microarray+path+protein+slides/pmc12455989-242-62-66
Average 86 stars, based on 1 article reviews
path protein microarray slides - by Bioz Stars,
2026-09
86/100 stars
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1) Product Images from "An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development"
Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development
Journal: Journal of Virology
doi: 10.1128/jvi.00478-25
Figure Legend Snippet: Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
Techniques Used: Microarray, Labeling, Fluorescence, Infection
Figure Legend Snippet: High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.
Techniques Used: High Throughput Screening Assay, Infection, Microarray, Virus
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The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped Article Title: Antibody Panel Based N-glycan Imaging for N-Glycoprotein Biomarker Discovery Article Snippet: Materials: IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. 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BP9706) Double distilled water PATH Protein Microarray Slides (Grace Biolabs, cat. no. 805025) ProPlate 4 Well Slide Modules (Grace Biolabs, cat. no 248864) Humidity chamber 1: 12×9×3.5cm western blot incubation box Kim Wipes (Kimberly-Clark, cat. no. 34155) Wypall X 60 paper towel (Kimberly-Clark, cat. no. 34790) Benchtop plate shaker Odyssey CLx Imager (Li-cor Biosciences) Image Studio software (Li-cor Biosciences) .. IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Blocking Assay:Article Title: Antibody Panel Based N-glycan Imaging for N-Glycoprotein Biomarker Discovery Article Snippet: Materials: IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Double distilled water PATH Protein Microarray Slides (Grace Biolabs, cat. no. 805025) ProPlate 4 Well Slide Modules (Grace Biolabs, cat. no 248864) Humidity chamber 1: 12×9×3.5cm western blot incubation box Kim Wipes (Kimberly-Clark, cat. no. 34155) Wypall X 60 paper towel (Kimberly-Clark, cat. no. 34790) Benchtop plate shaker Odyssey CLx Imager (Li-cor Biosciences) Image Studio software (Li-cor Biosciences) .. IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Western Blot:Article Title: Antibody Panel Based N-glycan Imaging for N-Glycoprotein Biomarker Discovery Article Snippet: Materials: IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Double distilled water PATH Protein Microarray Slides (Grace Biolabs, cat. no. 805025) ProPlate 4 Well Slide Modules (Grace Biolabs, cat. no 248864) Humidity chamber 1: 12×9×3.5cm western blot incubation box Kim Wipes (Kimberly-Clark, cat. no. 34155) Wypall X 60 paper towel (Kimberly-Clark, cat. no. 34790) Benchtop plate shaker Odyssey CLx Imager (Li-cor Biosciences) Image Studio software (Li-cor Biosciences) .. IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Incubation:Article Title: Antibody Panel Based N-glycan Imaging for N-Glycoprotein Biomarker Discovery Article Snippet: Materials: IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Double distilled water PATH Protein Microarray Slides (Grace Biolabs, cat. no. 805025) ProPlate 4 Well Slide Modules (Grace Biolabs, cat. no 248864) Humidity chamber 1: 12×9×3.5cm western blot incubation box Kim Wipes (Kimberly-Clark, cat. no. 34155) Wypall X 60 paper towel (Kimberly-Clark, cat. no. 34790) Benchtop plate shaker Odyssey CLx Imager (Li-cor Biosciences) Image Studio software (Li-cor Biosciences) .. IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Software:Article Title: Antibody Panel Based N-glycan Imaging for N-Glycoprotein Biomarker Discovery Article Snippet: Materials: IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Double distilled water PATH Protein Microarray Slides (Grace Biolabs, cat. no. 805025) ProPlate 4 Well Slide Modules (Grace Biolabs, cat. no 248864) Humidity chamber 1: 12×9×3.5cm western blot incubation box Kim Wipes (Kimberly-Clark, cat. no. 34155) Wypall X 60 paper towel (Kimberly-Clark, cat. no. 34790) Benchtop plate shaker Odyssey CLx Imager (Li-cor Biosciences) Image Studio software (Li-cor Biosciences) .. IRDye 800 CW Protein Labeling Kit (Li-cor Biosciences, cat. no. 928–38040) Target glycoprotein Target antibody or antibodies Phosphate buffered saline (PBS) Detergent wash solution (see recipe in Reagents and Solutions ) Octyl-β-D-glucopyranoside (Sigma-Aldrich, cat. no. O8001) BSA Blocking buffer (see recipe in Reagents and Solutions ) Bovine serum albumin (Fisher Scientific, cat. no. BP9706) Generated:Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development Article Snippet: .. Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on Affinity Purification:Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development Article Snippet: .. Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on Protein Array:Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates Article Snippet: Gold-coated GC-FP biosensor chips were fabricated as described previously., GC-FP chips were printed with an array of 400-μm diameter spots of target and control antigens/proteins using an ArrayIt SpotBot II microarray printer. .. Proteins/antigens were first diluted to 500 μg/mL in PBS and then further diluted 1:1 just prior to printing with Article Title: Multiplexed Detection and Quantification of Human Antibody Response to COVID-19 Infection Using a Plasmon Enhanced Biosensor Platform Article Snippet: Double-sided adhesive gaskets and acrylic or polycarbonate fluidic covers for the GC-FP biosensor chips were either fabricated at SUNY Polytechnic Institute or obtained from Ciencia, Inc. For use in the COVID-19 detection assay, GC-FP chips were printed with an array of approximately 400 μm diameter spots of target and control antigens/proteins using an ArrayIt SpotBot II microarray printer. .. All printed proteins/antigens were first diluted to 500 μg/μl in phosphate buffered saline (PBS) and then further diluted 1:1 just prior to printing with |
